PHYTOCHEMICAL ANALYSIS ON MORINGA OLEIFERA AND AZADIRACHTA INDICA LEAVES

ABSTRACT

Studies were conducted to determine the phytochemicals present in moringa oleifera and Azadirachta indica leaves. Leaves for this work were washed, room dried ground to powder. The ground leave for both sample were soaked in four different solvent; Ethanol, n-Hexane, Ethyl acetate and water for 24 hours. After the contact elapsed the solvent were filtered to recover the extract. Qualitative analysis was carried out on the extract; the result showed moringa oleifera to contain saponin, flavonoid, tannin, phenol, steroid and glycoside. Azadirachta indica contains; saponin, flavonoid, tannin, Alkaloid, steroid and glycoside. Quantitative analysis were also carried on the extract and the result showed moringa oleifera and Azadirachta indica to contain in percentage flavonoid (21.8 : 23.80), Alkaloids (5.00 : 8.20), saponin ( 0.70 : 1.10 ), phenol ( 0.76 : 1.49 ), Tannin ( 0.08 : 0.57 ) and Glycoside ( 0.005 : 0.0062 ) respectively for moringa oleifera and Azadirachta indica. In the qualitative analysis water was the best solvent for extraction and quantitative analysis; flavonoid, alkaloid, tannin, saponin, phenol and glycoside have higher percentage in Azadirachta indica than moringa oleifera.

TABLE OF CONTENT
Title page———————————————————————————i
Certification—————————————————————————–ii
Dedication——————————————————————————iii
Acknowledgement——————————————————————–iv
Abstract———————————————————————————-vi
Table of content———————————————————————-vii
List of figure—————————————————————————-xii
List of table—————————————————————————-xiv
CHAPTER ONE
1.1 Background of study————————————————————-1
1.2 Phytochemical———————————————————————3
1.2.1 Activity of phytochemical—————————————————-4
1.3 Statement of problem———————————————————–5
1.4 Aims and Objectives of study————————————————–6
1.5 Significant of study—————————————————————6
8
1.6 Scope of study———————————————————————6
CHAPTER TWO
2.0 Literature review——————————————————————7
2.1 Botany of Moringa oleifera —————————————————-7
2.1.1 Classification———————————————————————7
2.1.2 Origin and Habitat————————————————————–8
2.1.3 Description———————————————————————–9
2.1.4 Ecology and cultivation——————————————————-9
2.1.5 Traditional uses—————————————————————11
2.1.6 Common name—————————————————————-12
2.1.7 Pharmacological activity of Moringa oleifera ————————-13
2.2 Botany of Azadrichta indica ————————————————–18
2.2.1 Classification——————————————————————-18
2.2.2 Origin and habitat————————————————————19
2.2.3 Description———————————————————————19
2.2.4 Ecology and cultivation——————————————————21
9
2.2.5 Traditional uses—————————————————————22
2.2.6 Common name—————————————————————-22
2.2.7 Pharmacological activity of Azadrichta indica ————————-22
2.3 Phytochemical——————————————————————-26
2.3.1 Types of phytochemicals—————————————————-27
2.3.1.1 Tannin————————————————————————-28
2.3.1.2 Phenol————————————————————————-29
2.3.1.3 Saponin———————————————————————–30
2.3.1.4 Steroids———————————————————————–31
2.3.1.5 Flavonoids——————————————————————-33
2.3.1.6 Alkaloids———————————————————————-35
2.3.1.7 Glycosides——————————————————————–36
CHAPTER THREE
3.0 Materials and Methods——————————————————–37
3.1 Collection and preparation of material————————————37
3.2 Equipment / apparatus——————————————————–37
10
3.3 Reagents————————————————————————–38
3.4 Methods—————————————————————————39
3.4.1 Qualitative Analysis———————————————————-39
3.4.2 Preparation of Wagner’s reagent—————————————–40
3.4.3 Preparation of Mayer’s reagent——————————————-40
3.4.4 Test for Alkaloid—————————————————————40
3.4.5 Test for Saponin—————————————————————41
3.4.6 Test for Flavonoid————————————————————-42
3.4.7 Test for Steroid—————————————————————-42
3.4.8 Test for Tannin—————————————————————-42
3.4.9 Test for Glycoside————————————————————-43
3.5 Quantitative Analysis———————————————————–43
3.5.1 Flavonoid determination—————————————————-43
3.5.2 Alkaloid determination——————————————————44
3.5.3 Tannin determination——————————————————-44
3.5.4 Saponin determination——————————————————45
11
3.5.5 Glycoside determination—————————————————-45
3.5.6 Phenol determination——————————————————-46
CHAPTER FOUR
4.0 Results and Discussion———————————————————47
4.1 Qualitative Analysis result—————————————————-47
4.2 Quantitative Analysis result————————————————–49
4.3 Discussion of result————————————————————-50
CHAPTER FIVE
5.1 Conclusions———————————————————————–52
5.2 Recommendations————————————————————–52
REFERENCE——————————————————————53 – 54
APPENDIX——————————————————55 – 58
12
LIST OF FIGURES
Fig1Moringa oleifera leave———————————————————-7
Fig2Azadrichta indica leave——————————————————–18
Fig3 Conical flask ——————————————————————-59
Fig4 Water bath ———————————————————————59
Fig5 Electric thermal incubator ————————————————–60
Fig6 Test tube ————————————————————————60
Fig7 Electric stove ——————————————————————-61
Fig8 Separation process setup —————————————————61
Fig9 Separation funnel ————————————————————-62
Fig10 Thermostatic drying oven ————————————————-62
Fig11Beaker—————————————————————————63
Fig12 Measuring cylinder ———————————————————63
Fig13 Whatman filter paper ——————————————————64
Fig14 Constant temperature magnetic stirrer ——————————-64
13
Fig15 UV machine ——————————————————————-65
Fig16 Electronic weighing balance ———————————————-65
Fig17 phenol standard Graph—————————————————–66
14
LIST OF TABLE
Table 4.1a Qualitative Analysis Result of Moringa oleifera—————-47
Table 4.1b Qualitative Analysis Result of Azadrichta indica—————48
Table 4.2 Quantitative Analysis Result of Moringa oleifera and Azadirachta indica

CHAPTER ONE

1.1 BACKGROUND OF STUDY

From time immemorial, man depended on plants as medicine. From a historical perspective, it is evident that the fascination for plants is as old as mankind itself. The plant kingdom represent a rich storehouse of organic compounds, many of which have been used for medicinal purposes and could serve as lead for the development of novel agents having good efficacy in various pathological disorders in the coming years.Plants are the richest source of drugs for traditional medicine, modern medicines, nutraceuticals food supplements, folk medicine, pharmaceutical intermediates and chemical entities for synthetic drugs (Hammer et al., 1999). The use of plant product as medicines could be traced as far back as the beginning of human civilization. The earliest mentioned medicinal used plant in Hindu culture is found in “Rigveda”, which is said to have been written between 4500-1600 B.C. and is supposed to be the oldest repository human knowledge. The active principle isolated, have provided leads in the development of several life saving drugs, which are in use today (Rastogi and Mehrotra, 2002).

The isolated active compounds of the plants are secondary metabolites chemical compound that occur naturally in plant with no nutritional value to human life. These active compounds are generally called phytochemical. These phytochemicals play protective roles in plants, each chemical labeled phytochemical works in different ways, not all are the same for human, and not all come from the same plants. Some have shown more promise than others in fighting disease and illness in humans. There are some basic types of these active compounds that are found in different fruits and vegetables. We have some of them like antioxidants, they are present in onions and some other fruits and tea, they act as preventive measure for premature cell death and some forms of cancer and aging. Isoflavones or plant estrogen; they are found in soy and soy products; they are helpful in the year just before and after menopause. Capsaicin is found in hot pepper and it has been shown to significantly reduce prostate tumors in size, at least in mice. Taking capsaicin on a regular basis by eating spicy foods with hot peppers may prove an excellent preventative agent to prostate cancer and benign growth of prostate (Ahmedabad 382- 481). This experiment was carried out on moringa oleifera and Azadirachta Indica leaves.

Moringa Oleifera, or the horseradish tree, is a small or medium-sized about 10 m high perennial softwood tree with timber of low quality pantropical specie plant that is known by such regional name as benzolive, drumstick tree, kelor, marango, mlonge, mulangay, nebedaye, saikhan and sajan. Over the past two decades, many reports have appeared in the mainstream scientific journals describing its nutritional and medicinal properties (Akerele, 1993). Moringa Oleifera is the most widely cultivated species of a monogeneric family, the moringaceae that is native to the sub-Himalayan tracts of India, Pakistan Bangladesh and Afghanistan, it also now naturalizes in West Africa and Nigeria as a whole.

Azadirachta Indica on the other hand is a very useful traditional medicinal plant in the sub-continent and each part of the tree has some medicinal properties. The plant is native to Asia, but has now naturalized in West Africa and is widely cultivated in Nigeria as an ornamental as well as medicinal plant.

1.2 Phytochemicals

Phytochemicals are non-nutritive plant chemicals which occur naturally in plants that have protective or disease preventive properties. They are nonessential nutrients, meaning that they are not required by the human body for sustaining life. It is well-known that plant produces these chemicals to protect them but recent research demonstrates that they can also protect humans against diseases. There are more than thousand known phytochemicals. Some of the well-known phytochemicals are lycopene in tomatoes, isoflavones in soy and flavonoids in fruits.Alkaloids (examples are Caffeine, Theobromine, Theophylline).Organosulfides(examples are Allicin, Glutathione, Indole-3-Carbinol, Isothiocyanates).Tannins, steroids, Glycosides etc.

1.2.1 Activity of phytochemicals

Antioxidant – Most phytochemicals have antioxidant activity and protect our cells against oxidative damage and reduce the risk of developing certain types of cancer. Phytochemicals with antioxidant activity includes:allyl sulfides (onions, leeks, and garlic), carotenoids (fruits, carrots), flavonoids (fruits, vegetables), polyphenols (tea, grapes).

Hormonal action – Isoflavones, found in soy, imitate human estrogens and help to reduce menopausal symptoms and osteoporosis.Stimulation of enzymes – Indoles, which are found in cabbages, stimulate enzymes that make the estrogen less effective and thus could reduce the risk for breast cancer. Other phytochemicals, which interfere with enzymes, are protease inhibitors (soy and beans), terpenes (citrus fruits and cherries).

Interference with DNA replication – Saponins found in beans interfere with the replication of DNA cell, thereby preventing the multiplication of cancer cells. Capsaicin, found in hot peppers, protects DNA from carcinogens.

Physical action – Some phytochemicals bind physically to cell walls thereby preventing the adhesion of pathogens to human cell walls. Proanthocyanidins are responsible for the anti-adhesion properties of cranberry. Consumption of cranberries will reduce the risk of urinary tract infections and will improve dental health.

Phytochemicals are naturally present in many foods but it is expected that through bioengineering new plants will be developed, which will ontain higher levels. This would make it easier to incorporate enough phytochemicals with our food.

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