PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF FLOWER AND STALK OF MANGIFERA INDICA (OPIORO MANGO) CHEMISTRY Project Topics – Complete Project Material

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ABSTRACT Phytochemical and antimicrobial screening analysis of Mangifera indica indicated that the presence of the following moisture content 12.33% +0.0556%,  Alkaloid      7.35% +0.0291%, Tannins 18.22% +0.0070%, Saponic Glycoside 3.82% +0.0264%, Flanonoid  6.11% + 0.0158%, Phenolic glycoside 0.010% + 0.0012%, Terpenoids 5.71% + 0.01%, Antrachionic 10.59% + 0.0158%,  pH 3.84% + 0.0529%. Cardiac Glycoside  7.90% + 0.0234%. Extracts of Mangifera indica were investigated for its antifungal and antibacterial activities. The aqueous extract did not inhibit any of the test fungi, the ethanolic extract produced zones of inhibition against Staphylococcus aureus, S. pyogens, Escherichia coli, Bacilus subfilis, while C. albican and a. flavus were not inhibited.

TABLE OF CONTENT CONTENT                                                                             Title page                                                                               Declaration                                                                                               Certification                                                                                      Dedication                                                                                                 Acknowledgment                                                                                     Table of content                                                                             Abstract                                                                                           

CHAPTER ONE 1.0    INTRODUCTION                                                                            OBJECTIVE OF THIS RESEARCH                                    1.1    LITERATURE REVIEW                                                        1.2    GENERAL USES AND IMPORTANCE OF MANGIFERA INDICA                                                          1.3    MORPHLOGY OF MANGIFERA INDICA; THE TREE AND ITS FLOWERS                                                                                      CHAPTER TWO 2.0    MATERIALS AND METHODS                                            2.1    MATERIALS                                                                          2.2    EQUIPMENTS AND APPARATUS                                              2.3    CHEMICAL REAGENTS                                                      2.4    PHYTOCHEMICAL SCREENING (QUANTITATIVE        ANALYSIS)                                                                            2.5    DETERMINATION OF SAPONIN                                       2.6    DETERMINATION OF ALKALOID                                               2.7    DETERMINATION OF TANINS                                          2.8    DETERMINATION OF MOISTURE CONTENT                2.9    DETERMINATION OF TOTAL ASH                                   2.10  CARDIAC GLYCOSIDE                                                       2.11  TEST FOR ANTHRACENE GLYCOSIDES                       2.12  TERPENOIDS                                                                       2.13  pH AT 200C (10%)                                                                CHAPTER THREE TABLE 3.1 ORGANOLEPTIC  TEST  RESULT                          TABLE 3.2 RESULT OF THE  PHYTOCHEMICAL  ANALYSIS OF FLOWER AND STALK OF Mangifera Indica     TABLE 3.3RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO GRAM POSITIVE BACTERIA IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica                                                          TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF TWO GRAM NEGATIVE BACTERIA IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica                                                    TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF TWO FUNGI IN TWO CRUDE EXTRACTS OF FLOWER AND STALK OF Mangifera indica                                                    TABLE 3.6 RESULT OF MINIMUM INHIBITORY CONCENTRATION (MIC) OF CRUDE EXTRACT OF FLOWER AND STALK OF Mangifera indica                                                    CHAPTER FOUR DISCUSSION                                                              CONCLUSION                                                             RECOMMENDATION                                                 REFERENCES                                                            APPENDIX                                                                           

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